A rapid, simple, and sensitive liquid chromatography- tandem mass spectroscopy method was developed for quantification of Hydroxyzine and its active metabolite Cetirizine in human plasma using Quetiapine as internal standard. The analyte and internal standard was extracted by solid phase extraction using Orochem 30mg, 1ml catridge. Quantification was done on triple quadrapole mass spectrometer employing turbo ion spray mode. The separation of analyte and internal standard was done on Inertsil ODS-3, 100×4.6mm, 5µm column with mobile phase composition of 5mM Ammonium acetate (pH 4.0 adjusted with formic acid): Methanol: Acetonitrile in the ratio of 5:5:90 v/v/v. The total chromatographic runtime was 3min. The linearity range for Hydroxyzine and Cetirizine was 0.250ng/ml-100.093ng/ml, 1.000ng/ml-501.179ng/ml respectively. This method was fully validated for its sensitivity, accuracy, precision, linearity, recovery, matrix effect, dilution integrity, and stability studies.
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